Metallic copper nanoparticles modified glassy carbon electrode is fabricated by reduction of CuSO4 in the presence of cetyltrimethylammonium bromide (CTAB) through potentiostatic method. As-prepared nanoparticles are characterized by scanning electron microscopy and electrochemical methods. Copper oxide modified glassy carbon electrode (nano-CuO/MGCE) is prepared using consecutive potential scanning in 0.1 M NaOH solution. The electrochemical properties of hydrazine is studied onto either nano-CuO/MGCE or bare GCE by using cyclic voltammetry and chronoamperometry techniques. The results show that the nano-CuO/MGCE can catalyze the hydrazine oxidation in alkaline medium. The electrocatalytic oxidation peak current shows linear dependency on hydrazine concentration. Linear analytical curves are obtained in the ranges of 0.025-1.66 mM and 0.05-2.5 mM by using differential pulse voltammetry (DPV) and amperometry methods, respectively. The detection limits (3s) are determined as 2×10-5 M and 1.2×10-5 M by using amperometry and DPV methods, respectively. The Catalytic rate constant is estimated by using chronoamperometry method. Stability of the modified electrode has also been investigated.